Abstract: A new design method of a macro-biological pressure-retaining sampling-culture system at full-ocean depth(operating water depth of 11 000 m) was presented. The system might realize macro-biological pressure-retaining sampling in the full-ocean-depth environment, and at the same time met the requirements of pressure-free transferring and in-situ culture in the laboratory. The basic structure and working principles of the full-ocean-depth macro-biological pressure-retaining sampling-culture system were introduced in details, and the full-ocean-depth macro-biological pressure-retaining sampling-culture system were designed and analyzed. With the developed engineering prototype of the full-ocean-depth macro-biological pressure-retaining sampling, the pressure-retaining performance and sample transfer tests of the full-ocean-depth macro-biological pressure-retaining sampling were carried out. The experimental results show that the full-ocean-depth macro-biological pressure-retaining sampling may bear the pressure of 127 MPa, and the greater the internal pressure of the sampler, the better the pressure-retaining performance, and the transfer without pressure drop is realized in the ultra-high pressure environments, to provide high-quality macro-biological pressure-retaining samples for deep seabed scientific research.

Key words: deep sea environment, pressure-retaining sampling, pressure-retaining transferring, in situ culture

摘要： 提出了一种新型的全海深（作业水深11000 m）宏生物保压采样培养系统的设计方法。该系统能够实现全海深环境下宏生物保压采样，同时满足实验室无压降转移和原位培养。详细介绍了全海深宏生物保压培养系统的基本结构及工作原理，对全海深宏生物保压采样培养系统进行了设计与分析。利用研制的全海深宏生物保压采样器工程样机开展了全海深宏生物保压采样器保压性能和样品转移试验。试验结果表明，全海深宏生物保压采样器能够承载127 MPa的压力，压力越大，采样器保压性能越好，且在超高压环境下实现了无压降转移,为深渊海底科学研究提供了高质量的宏生物保压样品。

关键词: 深海环境, 保压采样, 保压转移, 原位培养