Because the stiffness curve of robotic leg has a certain deviation from the joint or limb stiffness, the structure design method of robotic leg was proposed based on biological joint or limb stiffness. Firstly, according to the principle of virtual work, the equation of joint or limb stiffness curve which could be obtained by using curve interpolation, the structure design method of robotic leg was obtained based on joint or limb stiffness. Then, integrated simulation and structure optimization of rigid-flexible coupling robotic leg were implemented based on Patran, Nastran and Adams. After that, according to the basic parameters of the human and the relevant parameter of ankle stiffness during human hopping, the structure design and its optimization were implemented respectively, at the same time, the simulation results were given to prove the effectiveness and advantages of the mothed.