In order to reduce the assembly work fatigue, reduce the intensity of action and improve the assembly quality and efficiency, a comprehensive evaluation model was proposed to evaluate feasible assembly sequences objectively based on human factor simulation analysis. Taking human factor performance of assembly sequences as the goal, a comprehensive evaluation index system including quantitative and qualitative indicators was created as well as human factor engineering simulation analysis model for assembly sequences was proposed, on the basis of it, the attribute value of basic index could be obtained by virtual simulation technology and human factor analysis method.The weights of quantitative index and qualitative index were determined by adopting objective and adaptive entropy method and fuzzy analytic hierarchical process respectively, qualitative evaluation value was quantified using triangular fuzzy number, then based on Topsis framework, the optimal assembly sequence can be drawn. Finally, the evaluation model was applied to a aerospace product reducer feasible assembly sequence optimization combined with Tecnomatix digital simulation platform, verifying its validity.